Life Science / Technology Services Ι MLPA analysis

MLPA analyses

Multiplex ligation-dependent probe amplification (MLPA) is a method of presenting copy number variations (CNV) of up to 50 target sequences in a single reaction. The amplified products are analysed by means of electrophoresis. Because only one primer pair is used to amplify all PCR products, the fragment analysis is robust and highly reproducible. The fragments are compared quantitatively with a control sample in order to identify any changes in the copy number of the target sequence. MLPA can also be used for quantitative methylation analysis.